Recombinant Human IL17A

• Description:

  The founding member of the IL17 family of cytokines, IL17A plays an important role in antimicrobial and chronic inflammation. All the six IL17 cytokines (IL17A-F) share a cystine knot fold and are secreted as homodimers. Mature human IL17A shares 60% amino acid sequence identity with rat and mouse IL17A. IL17 cytokines bind to two receptors; IL17RA and IL17RC that together form a heterodimer. The receptor heterodimer is preferred by IL17 ligands, and thus, either IL17RA or IL17RC knockouts completely abolish IL17 signalling. Recombinant human IL17A is a non-glycosylated, 32 kDa disulfide-linked homodimer.

• Gene Name:

  IL17A

• Gene Aliases:

  IL-17, CTLA-8, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8
  Recombinant Human Interleukin-17A (IL17A)

• Gene ID:

  3605

• Accession Number:

  Q16552

• Organism:

  Human

• Source:

  E. coli

• Concentration:

  <1.0 EU/μg of recombinant protein as determined by the LAL method

• Purity:

  >95% as determined by SDS-PAGE

• Solubility:

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

• Function:

  The ED(50), as determined by the dose-dependent proliferation of induction of IL6 in primary human foreskin fibroblasts was found to be approximately 2.0 ng/mL, corresponding to a specific activity of > 5.0 x 10^5 units/mg

• Molecular Weight:

  16

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance:

  Lyophilized Powder

• Formulation:

  Lyophilized from a 0.2 μm filtered solution in PBS (pH 7.5)

• CAS Number:

  9000-83-3