Recombinant Human IFNA2

Recombinant Human IFNA2

• Description: All known subtypes of IFN- alpha show the same antiviral antiparasitic, antiproliferative activities. Human IFN-alpha is also a potent antiviral substance in murine, porcine, and bovine cell systems. IFN-alpha forms are produced by monocytes/macrophages, lymphoblastoid cells, fibroblasts, and a number of different cell types following induction by viruses, nucleic acids, glucocorticoid hormones, and low-molecular weight substances. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. IFN-alpha and IFN-beta are thought to bind to the same receptor. Signal transduction mechanisms elicited after binding of IFN-alpha to its receptors involves tyrosine phosphorylation (see also: PTK; protein tyrosine kinase) of various non-receptor tyrosine kinases belonging to the Janus kinases.
• CAS Number: 9000-83-3
• Gene Name: IFNA2
• Gene Aliases: IFNA2A, IFN-alpha-2a, LeIF A
  Recombinant Human Interferon-Alpha 2A (IFNA2)
• Gene ID: 3440
• Accession Number: P01563
• Organism: Human
• Source: E. coli
• Concentration: <1.0 EU/μg of recombinant protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE
• Solubility: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Function: The ED(50) was determined by the dose-dependent anti-proliferation of Ovcar3 cells and was found to be &lt;20ng/mL.
• Molecular Weight: 19
• Shipping Conditions: Ambient Temperature
• Storage Conditions: The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
• Appearance: Lyophilized Powder
• Formulation: Recombinant Interferon-alpha 2A was lyophilized from a 0.2 μm filtered PBS solution pH 7.0.