Recombinant Human GDNF

• Description :

  GDNF is a disulfide-bonded homodimeric glycosylated protein of 134 amino acids. The amino acid sequences inferred from rat and human cDNAs are 93% identical. The proteins contain seven conserved cysteine residues in the same relative spacing found in all members of the TGF-beta superfamily of proteins. In embryonic midbrain cultures GDNF promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

• Gene Name :

  GDNF

• Gene Aliases :

  GDNF, Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF
  Recombinant Human Glial-Derived Neurotrophic Factor (GDNF)

• Accession Number :

  P39905

• Organism :

  Human

• Source :

  E. coli

• Concentration :

  <1.0 EU/μg of recombinant protein as determined by the LAL method.

• Purity :

  >95% as determined by SDS-PAGE

• Solubility :

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

• Function :

  The ED(50) was determined by the proliferation of rat C6 cells is < 0.1 ng/mL, corresponding to a specific activity of > 1.0 x 10^7 units/mg.

• Molecular Weight :

  15

• Shipping Conditions :

  Ambient Temperature

• Storage Conditions :

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance :

  Lyophilized Powder

• Formulation :

  Recombinant Glial-Derived Neurotrophic Factor was lyophilized from a 0.2 μm filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween80, 5 mM Glutamic acid, pH 4.5.

• CAS Number :

  9000-83-3