Recombinant Human Fractalkine (CX3CL1)

• Description:

  Fractalkine is a type I membrane protein that signals through the CX3CR1 receptor. The extracellular domain of Fractalkine is released by protease cleavage – producing soluble Fractalkine. Fractalkine has been found in many tissues and shown to be up-regulated in endothelial cells and microglia in response to inflammatory signals. Fractalkine is also involved in leukocyte adhesion as well as attracting monocytes, microglia cells and NK cells.

• Gene Name:

  CX3CL1

• Gene Aliases:

  C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1

• Gene ID:

  6376

• Accession Number:

  P78423

• Organism:

  Human

• Source:

  E. coli

• Concentration:

  <1.0 EU/μg of recombinant protein as determined by the LAL method.

• Purity:

  >95% as determined by SDS-PAGE

• Solubility:

  A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

• Function:

  Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CX3CR1. The ED(50) for this effect is typically 20­100 ng/mL

• Molecular Weight:

  8.9

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance:

  Lyophilized Powder

• Formulation:

  Lyophilized from a 0.2μm filtered solution in 20mM phosphate buffer and 50mM NaCl, pH 7.4

• CAS Number:

  9000-83-3