Recombinant Human FGF2

CAT: 0171-Z101457Size: 500 µgDry Ice: NoHazardous: No
CAT#:0171-Z101457Size:500 µg
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Description
Basic Fibroblast Growth Factor is found in almost all tissues of mesodermal and neuroectodermal origin and also in tumors derived from these tissues. Endothelial cells produce large amounts of this factor. Some bFGF is associated with the extracellular matrix of the subendothelial cells. Many cells express bFGF only transiently and store it in a biologically inactive form. The mechanism by which the factor is released by the cells is not known. It is released after tissue injuries and during inflammatory processes. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation. bFGF stimulates the growth of fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, and many other cell types.
CAS Number
9000-83-3
Gene Name
FGF2
Gene Aliases
bFGF, Fibroblast growth factor 2, FGF-2, Heparin-binding growth factor 2, HBGF-2
Recombinant Human Basic Fibroblast Growth Factor (FGF2)
Gene ID
2247
Accession Number
P09038
Organism
Human
Source
E. coli
Concentration
< 1.0 EU/μg of recombinant protein as determined by the LAL method.
Purity
>95% as determined by SDS-PAGE
Solubility
A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL is recommended. Please note, filter sterilization is a must following reconstitution. This solution can then be diluted into other buffers.
Function
The bioactivity was determined in a NIH/3T3 cell proliferation assay. The ED50 was in the range of 0.05 - 0.5 ng/ml.
Molecular Weight
17
Shipping Conditions
Ambient Temperature
Storage Conditions
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Appearance
Lyophilized Powder
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS

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