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Recombinant Human CXCL14 (BRAK)

CAT: 0171-Z102375Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0171-Z102375Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Chemokine (CXC motif) ligand 14, also known as breast and kidney-expressed chemokine (BRAK), is a CXC chemokine that is a potent chemoattractant for neutrophils but not for T-cells, B-cells, monocytes or granulocytes. Mature CXCL14 has many conserved features of the CXC family and is 30% homologous to the MIP-α and MIP-β sequences, but differs in that it has an additional five amino acid sequence in between the third and fourth cysteines and a short N-terminus. It is usually highly expressed near its fibroblast source and in epidermal fibroblasts and keratinocytes of skin, but is absent from cancer cells.
CAS Number
9000-83-3
Gene Name
CXCL14
Gene Aliases
Chemokine BRAK, MIP-2G, Small-inducible cytokine B14
Gene ID
9547
Accession Number
O95715
Organism
Human
Source
E. coli
Concentration
<1.0 EU/μg of recombinant protein as determined by the LAL method
Purity
>95% as determined by SDS-PAGE
Solubility
A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Function
The ED50 is 0.25-1.25 ng/mL as determined by its ability to induce calcium flux in activated monocytes
Molecular Weight
9.6
Shipping Conditions
Ambient Temperature
Storage Conditions
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Appearance
Lyophilized Powder
Formulation
Lyophilized from a 0.2µm filtered solution containing 20mM Tris pH 8.5 and 1M NaCl

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