Recombinant Human AIMP1

• Description:

  EMAP-II is activated during apoptosis by cleavage of pro-EMAP through caspase 8 and enhances the recruitment of inflammatory cells by its chemotactic properties. Besides its chemotactic effect on inflammatory cells, EMAP-II increases the adhesiveness of coronary artery smooth muscle cells (CASMC) for monocytes. EMAP-II also has anti-angiogenic properties, which are explained by its pro-apoptotic effect on endothelial cells.

• Gene Name:

  AIMP1

• Gene Aliases:

  EMAP II, EMAP-2, Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, Multisynthase complex auxiliary component p43, Small inducible cytokine subfamily E member 1, SCYE1
  Recombinant Human Endothelial Monocyte-Activating Polypeptide 2 (AIMP1)

• Gene ID:

  9255

• Accession Number:

  Q12904

• Organism:

  Human

• Source:

  E. coli

• Concentration:

  <1.0 EU/μg of recombinant protein as determined by the LAL method.

• Purity:

  >95% as determined by SDS-PAGE

• Solubility:

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

• Function:

  The ED(50) was determined by apoptotic effect on MCF-7 cells using a concentration of 10-50 ng/mL.

• Molecular Weight:

  18

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance:

  Lyophilized Powder

• Formulation:

  Lyophilized from a 0.2 μm filtered 20 mM PBS,130 mM NaCl solution pH 7.5

• CAS Number:

  9000-83-3