Anti-TNF alpha(J1D9), CF740 conjugate

Anti-TNF alpha(J1D9), CF740 conjugate

• Description: This antibody neutralizes TNF alpha biological activities. It prevents TNF alpha induced apoptosis in Jurkat cells. It also neutralizes HurTNFamediated cytotoxicity of L929 cells and inhibits tumor growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: APC1; Cachectin; Differentiation inducing factor (DIF); Macrophage cytotoxic factor (MCF); Necrosin; TNF alpha; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precursor
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: TNF
• Gene ID: 7124
• NCBI Gene ID: 241570
• UniProt: P01375
• Cellular Locus: Secreted (extracellular)
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant human TNF-alpha
• Target Antigen: TNF-Alpha
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: J1D9
• Conjugation: CF740
• Source: Animal
• Applications: Functional studies (published) | IHC, FFPE (published) | ELISA (published)
• Validated Applications: IHC, FFPE, ELISA
• Field of Research: Cytokines, Immunology
• Positive Control: HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% rBSA, 0.05% azide
• Molecular Weight: 17 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Does not react with mouse; others not known
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Immunol Cell Biol (1990) 68(1): 51-55. (ELISA) Anticancer Research, 30 Jun 1992, 12(4):1243-1246. (functional studies) Dig Dis Sci (2010) 55:617-625. (IHC, FFPE)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years