Anti-ASRGL1 (Asparaginase Like 1) (CRASH/1289), CF405S conjugate

Anti-ASRGL1 (Asparaginase Like 1) (CRASH/1289), CF405S conjugate

• Description: ASRGL1 (Asparaginase-like protein 1), also known as CRASH, is a 308 amino acid protein belonging to the Ntn-hydrolase family. Asparaginases utilize asparagine as a substrate to produce aspartic acid and ammonia. ASRGL1 has been identified as a autoantigenic protein that is present in the mid-piece of sperm after obstruction of the male reproductive tract. ASRGL1 is expressed highly in testis, but is also expressed in brain, kidney and gastrointestinal tissues. High levels of ASRGL1 have also been identified in ovarian, uterine and mammary tumors in comparison with normal tissues of the same origin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: ASRGL1; isoaspartyl peptidase; L-asparaginase; ALP1; beta-aspartyl-peptidase; CRASH; L asparagine amidohydrolase
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: ASRGL1
• Gene ID: 80150 (Human)
• NCBI Gene ID: 535326 (Human)
• UniProt: Q7L266 (Human)
• Cellular Locus: Cytoplasmic|Cytoskeleton|Nucleus
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant human full-length ASRGL1
• Target Antigen: ASRGL1
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: CRASH/1289
• Conjugation: CF405S
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Cancer, Metabolism
• Positive Control: HeLa cells. Fallopian Tube, Endometrium, Uterus or Cervix.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 17-25 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin): 0.5-1 ug/mL|Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years