GMP Human GM-CSF Protein

GMP Human GM-CSF Protein

• Background: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also known as Colony stimulating factor 2 (granulocyte-macrophage), is a cytokine initially characterized by its ability to induce colonies of granulocytes and macrophages from myeloid progenitor cells, and is secreted by macrophages, T cells, mast cells, endothelial cells and fibroblasts. GM-CSF is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. Monocytes exitthe circulation and migrate into tissue, whereupon they mature into macrophages and dendritic cells. Thus, it is part of the immune/inflammatory cascade, by which activation of a small number of macrophages can rapidly lead to an increase in their numbers, a process crucial for fighting infection. The active form of the protein is found extracellularly as a homodimer. Human GM-CSF glycosylated in its mature form. As a part of the immune/inflammatory cascade, GM-CSF promotes Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity, and thus worthy of consideration for therapeutic target. GM-CSF has also recently been evaluated in clinical trials for its potential as a vaccine adjuvant in HIV-infected patients. The preliminary results have been promising. GM-CSF is also used as a medication to stimulate the production of white blood cells following chemotherapy.
• Description: GMP Human GM-CSF Protein (GMP-GMFH28) is expressed from human 293 cells (HEK293). It contains AA Ala 18 - Glu 144 (Accession # P04141).
• Specifications: This protein carries no "tag". The protein has a calculated MW of 14.5 kDa. The protein migrates as 25 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Ala 18 - Glu 144
• Host: HEK 293
• Target: GM-CSF
• Conjugation: Unconjugated
• Tag: Native
• Source: Human
• Stability: -20°C to -70°C for 5 years in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution._x000D_ Upon receipt, store it immediately at -20°C or lower for long term storage.
• Endotoxin: 10 EU/mg
• Purity: 95%
• Format: Powder-GMP
• Buffer: PBS, pH7.4
• Molecular Weight: 14.5 kDa
• Shipping Conditions: Blue ice
• Storage Conditions: -20°C