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Vimentin (LN-6), 1mg/mL

CAT: 0037-BNUM0060-50Size: 1x 50 µLDry Ice: NoHazardous: No
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Description
This MAb reacts with a 58 kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin. Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.This antibody is available purified, with BSA (0.2 mg/mL) or purified, BSA-free (1 mg/mL) .
CAS Number
9007-83-4
Synonyms
VIM
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
VIM
Gene ID
7431
NCBI Gene ID
455493
UniProt
P08670
Swiss Prot
P08670
Host
Mouse
Species Reactivity
Cat, Cow, Human, Mouse, Pig, Rabbit, Rat, Sheep
Reactivity
Vimentin
Immunogen
Human thymic nuclear extract
Target Antigen
Vimentin
Clonality
Monoclonal
Clone
LN-6
Type
Primary
Source
Animal
Origin
Animal
Applications
IF (published) |IHC, frozen (published) |IHC, FFPE (verified) |IP (published) |WB (published)
Field of Research
Cancer, Cytoskeleton
Positive Control
Jurkat cells, Sarcomas, Melanomas
Stability
2 years
Concentration
1 mg/mL
Buffer
PBS, no BSA, no azide
Molecular Weight
57-60 kDa
References & Citations
Note: References for this clone sold by other suppliers may be listed for expected applications. J Histochem Cytochem (1989 ) 37(9) 1363-1370. (IHC, FFPE; IHC, frozen; IF; IP) Int J Toxicol (2007) 26:289-295. (IHC; WB) Stem Cell Res Ther (2018) 9: 178 (Flow, intracellular)
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgM
Species
Mus musculus (mouse)
Classification
PBS, no BSA, no sodium azide (CAS 26628-22-8)
Conjugate
Purified, BSA-free

UniProtKB · P08670

Vimentin

VIME_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P08670
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
VIM
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B0YJC2, D3DRU4, Q15867, Q15868, Q15869, Q548L2, Q6LER9, Q8N850, Q96ML2, Q9NTM3
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationGlycoproteinIsopeptide bondPhosphoproteinS-nitrosylationUbl conjugation

Disease

CataractDisease variant

Cellular component

Cell membraneCell projectionCytoplasmCytoskeletonIntermediate filamentMembraneNucleus

Domain

Coiled coil

Biological process

Host-virus interaction
Product Datasheet
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