Anti-Vimentin(LN-6)

• Description:

  This MAb reacts with a 58 kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin. Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.This antibody is available purified, with BSA (0.2 mg/mL) or purified, BSA-free (1 mg/mL).

• Synonyms:

  VIM

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  VIM

• Gene ID:

  7431

• NCBI Gene ID:

  455493

• UniProt:

  P08670

• Cellular Locus:

  Cytoskeleton

• Host:

  Mouse

• Species Reactivity:

  Cat, Cow, Human, Mouse, Pig, Rabbit, Rat, Sheep

• Immunogen:

  Human thymic nuclear extract

• Target Antigen:

  Vimentin

• Clonality:

  Monoclonal

• Isotype:

  IgM

• Clone:

  LN-6

• Conjugation:

  Purified, with BSA

• Source:

  Animal

• Applications:

  IF (published) | IHC, frozen (published) | IHC, FFPE (verified) | IP (published) | WB (published)

• Validated Applications:

  IF, IHC, IHC, FFPE, IP, WB

• Field of Research:

  Cancer, Cytoskeleton

• Positive Control:

  Jurkat cells, Sarcomas, Melanomas

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  57-60 kDa

• Additionnal Information:

  Immunohistology formalin-fixed 1-2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. J Histochem Cytochem (1989 ) 37(9) 1363-1370. (IHC, FFPE; IHC, frozen; IF; IP) Int J Toxicol (2007) 26:289-295. (IHC; WB) Stem Cell Res Ther (2018) 9: 178 (Flow, intracellular)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4