Anti-VEGF-A(VEGF/1063)

• Description:

  This MAb recognizes proteins of 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  MVCD1; Vascular Endothelial Growth Factor A (VEGF-A); Vascular Permeability Factor (VPF); VEGFA

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  VEGFA

• Gene ID:

  7422

• NCBI Gene ID:

  73793

• UniProt:

  P15692

• Cellular Locus:

  Plasma membrane|Secreted (extracellular)

• Host:

  Mouse

• Species Reactivity:

  Dog, Human, Mouse, Rabbit, Rat

• Immunogen:

  Human VEGF189 recombinant protein

• Target Antigen:

  VEGF-A

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  VEGF/1063

• Conjugation:

  Purified, with BSA

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Angiogenesis, Cancer

• Positive Control:

  Tumor cells in astrocytomas, breast or ovarian carcinomas.

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing)

• Additionnal Information:

  Immunohistology formalin-fixed 2-4 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Immunofluorescence 1-2 ug/mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4