Anti-TTF-1 / NKX2.1(8G7G3/1), CF405S conjugate

Anti-TTF-1 / NKX2.1(8G7G3/1), CF405S conjugate

• Description: Recognizes a protein of 40 kDa, identified as Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates.Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: BCH|Benign chorea|BHC|Homeobox protein Nkx2.1|NK2 homeobox 1|NKX2.1|NKX2A|TEBP|Thyroid nuclear factor-1|Thyroid specific enhancer binding protein|Thyroid transcription factor-1 (TTF-1)|Tin man|TITF1|TTF-1
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: NKX2-1
• Gene ID: 7080
• NCBI Gene ID: 94367
• UniProt: P43699
• Cellular Locus: Nucleus
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Rat full length TTF-1 recombinant protein
• Target Antigen: NKX2.1 | TTF-1
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: 8G7G3/1
• Conjugation: CF405S
• Disease: Tumor
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Cancer, Transcription factors
• Positive Control: MAD109, MLE-15, H441-4, or H345 cells. Normal thyroid or lung.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 40 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Predicted to show broad species reactivity|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years