Anti-TNF alpha(4C6-H8)

Anti-TNF alpha(4C6-H8)

• Description: Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.
• Synonyms: APC1; Cachectin; Differentiation inducing factor (DIF); Macrophage cytotoxic factor (MCF); Necrosin; TNF alpha; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precursor
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: TNF
• Gene ID: 7124
• NCBI Gene ID: 241570
• UniProt: P01375
• Cellular Locus: Secreted (extracellular)
• Host: Mouse
• Species Reactivity: Cat, Dog, Human, Mouse, Rabbit, Rat, Zebrafish
• Immunogen: A hexadecapeptide corresponding to aa115-130 (NGVELRDNQLVVPSEG) of human TNF-?, conjugated to thyroglobulin
• Target Antigen: TNF-Alpha
• Clonality: Monoclonal
• Isotype: IgM κ
• Clone: 4C6-H8
• Conjugation: Purified, BSA-free
• Source: Animal
• Applications: IF (verified) | IHC, FFPE (verified)
• Validated Applications: IF, IHC, FFPE
• Field of Research: Cytokines, Immunology
• Positive Control: HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: 17 kDa
• Additionnal Information: Immunohistology formalin-paraffin 2-4 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years