Anti-Thyroglobulin(6E1.), CF488A conjugate

Anti-Thyroglobulin(6E1.), CF488A conjugate

• Description: Thyroglobulin is a 660 kDa dimeric pre-protein with mutiple glycosylation sites. It is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. Prior to forming dimers, thyroglobulin monomers undergo conformational maturation in the endoplasmic reticulation. The vast majority of follicular carcinomas of the thyroid will give positive immunoreactivity for anti-thyroglobulin even though sometimes only focally. Poorly differentiated carcinomas of the thyroid are frequently anti-thyroglobulin negative. Adenocarcinomas of other-than-thyroid origin do not react with this antibody. This antibody is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of thyroglobulin in metastatic lesions establishes the thyroid origin of tumor. Anti-thyroglobulin, combined with anti-calcitonin, can identify medullary carcinomas of the thyroid. Furthermore, anti-thyroglobulin, combined with anti-TTF1, can be a reliable marker to differentiate between primary thyroid and lung neoplasms.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: AITD3; hTG; TDH3; Tg; Tgn
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: TG
• Gene ID: 7038
• NCBI Gene ID: 654591
• UniProt: P01266
• Cellular Locus: Secreted (extracellular)
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Human thyroid follicular cells
• Target Antigen: Thyroglobulin
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: 6E1.
• Conjugation: CF488A
• Disease: Tumor
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Cancer
• Positive Control: Thyroid
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 660 kDa (Dimeric Form)
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Does not react with mouse or rat, others not known|Immunohistology formalin-fixed 0.1-0.2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years