Anti-TGFalpha(1E8-G6)

Anti-TGFalpha(1E8-G6)

• Description: This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF-beta (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.
• Synonyms: EGF-like TGF; ETGF; TFGA; TGF Type 1; TGFA; Wa1; Waved 1
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: TGFA
• Gene ID: 7039
• NCBI Gene ID: 170009
• UniProt: P01135
• Cellular Locus: Secreted (extracellular)
• Host: Mouse
• Species Reactivity: Human, Rabbit, Zebrafish
• Immunogen: A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGF?.
• Target Antigen: TGF-Alpha
• Clonality: Monoclonal
• Isotype: IgM κ
• Clone: 1E8-G6
• Conjugation: Purified, with BSA
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Signal transduction
• Positive Control: Jurkat or Ramos cells. Testicular Carcinoma
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 6 kDa
• Additionnal Information: Immunohistology formalin-paraffin 2-4 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 1-2 ug/mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years