Anti-TAG-72 / CA72.4(B72.3)

Anti-TAG-72 / CA72.4(B72.3)

• Description: Recognizes an oncofetal antigen of 220 kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: CA72.4, Tumor associated glycoprotein 72
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: tag-72
• Gene ID: 182875
• NCBI Gene ID: Not Known
• UniProt: Not Known
• Cellular Locus: Cytoplasmic|Plasma membrane
• Host: Mouse
• Species Reactivity: Cow, Dog, Hamster, Human, Rat
• Immunogen: Membrane-enriched fraction of a human breast carcinoma liver metastasis
• Target Antigen: CA72.4 | TAG-72
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: B72.3
• Conjugation: Purified, with BSA
• Disease: Tumor
• Source: Animal
• Applications: Flow, surface (published) | IHC, FFPE (verified) | IP (published) | RIA (published) | WB (published)
• Validated Applications: FC, IHC, FFPE, IP, RIA, WB
• Field of Research: Cancer
• Positive Control: Jurkat cells, Breast or lung carcinoma.
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 220 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. PNAS USA (1981) 78(5): 3199-3203. (IHC, FFPE; RIA) Clin Exp Metastasis (2012) 29:229-238. (Flow, surface; IP; WB)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years