Anti-S100B(4C4.9 + S100B/1012)

Anti-S100B(4C4.9 + S100B/1012)

• Description: S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein._x000D__x000D_Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: NEF|Protein S100-B|S-100 protein beta chain|S100 calcium binding protein beta (neural)|S100 calcium-binding protein B|S100 protein beta chain|S100B|S100beta
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: S100B
• Gene ID: 6285
• NCBI Gene ID: 422181
• UniProt: P04271
• Cellular Locus: Cytoplasmic|Nucleus|Vesicular
• Host: Mouse
• Species Reactivity: Cow, Human, Mouse, Rat
• Immunogen: Purified bovine brain S100 protein (4C4.9); Recombinant full-length human S100B protein (S100B/1012)
• Target Antigen: S100B
• Clonality: Monoclonal
• Isotype: IgG2a κ
• Clone: 4C4.9 S100B/1012
• Conjugation: Purified, BSA-free
• Disease: Tumor
• Source: Animal
• Applications: IF (verified) | IHC, FFPE (verified)
• Validated Applications: IF, IHC, FFPE
• Field of Research: Cancer
• Positive Control: Brain, Melanoma
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: 10-12 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.25-0.5 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years