Anti-Pmel17 / gp100 / SILV(NKI-beteb)

• Description:

  By immunohistochemistry, this antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  95kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanoma associated ME20 antigen, Melanosomal matrix protein17, p100, p26, PMEL17, Premelanosome protein, Secreted melanoma-associated ME20 antigen, SILV, Silver homolog

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  PMEL

• Gene ID:

  6490

• NCBI Gene ID:

  95972

• UniProt:

  P40967

• Cellular Locus:

  Endoplasmic reticulum|Golgi apparatus

• Host:

  Mouse

• Species Reactivity:

  Horse, Human

• Immunogen:

  Membranes from a human melanoma metastasis

• Target Antigen:

  gp100 | Pmel17 | SILV

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  NKI-beteb

• Conjugation:

  Purified, BSA-free

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  Flow, intracellular (published) | IF (published) | IHC, frozen (published) | ELISA (published) | IHC, FFPE (verified) | IP (published)

• Validated Applications:

  FC, IF, IHC, ELISA, IHC, FFPE, IP

• Field of Research:

  Cancer

• Positive Control:

  SK-MEL-28 cells or Melanoma

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  90-100 kDa

• Additionnal Information:

  Immunohistology formalin-fixed 1-2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 1-2 ug/mL|Does not react with rat, others not tested|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Am J Pathol (1988) 130(1): 179. (mAb characterization; IHC, FFPE; IF; ELISA; IP; WB) Am J Pathol (1993) 143(6): 1579. (IHC, frozen; IF) J Biol Chem (2004) 279(27): 28330-28338. (epitope mapping; IP; reports that NKI-beteb does not work well for WB) Exp Dermatol (2010) 19(8): e282-e285. (IF; Flow, intracellular)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4