Anti-PD1 / PDCD1 / CD279(PDCD1/922), CF640R conjugate

• Description:

  PD-1 (also known as Programmed Death 1, CD279, and PDCD1) is a negative regulatory T cell surface receptor. PD-1 is a Type I transmembrane protein expressed on the plasma membrane of T-cells. It is a T-cell checkpoint protein, involved in preventing autoimmunity. Because binding of PD-1 by its ligands PD-L1 or PD-L2 has a suppressive effect on the immune system, inhibition of the PD-1/PD-L1 pathway is a major strategy of immune-oncology therapies. PD-L1 is upregulated in certain cancers, and blocking PD-1 or PD-L1 can inhibit cancer growth._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

• Synonyms:

  CD279; hPD-1; hSLE1; PD1; PDCD1; Programmed Cell Death Protein 1; Protein PD-1; SLEB2; Systemic lupus erythematosus susceptibility 2

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  PDCD1

• Gene ID:

  5133

• NCBI Gene ID:

  158297

• UniProt:

  Q15116

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant full-length human PDCD1 protein

• Target Antigen:

  CD279 | PD1 | PDCD1

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  PDCD1/922

• Conjugation:

  CF640R

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  Flow (verified) | IF (verified) | IHC, FFPE (verified)

• Validated Applications:

  FC, IF, IHC, FFPE

• Field of Research:

  Apoptosis, Cancer, Cancer immunotherapy/immune checkpoint

• Positive Control:

  TY cells or Tonsil.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  55 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4