Anti-PCNA(PCNA/694), CF405S conjugate

Anti-PCNA(PCNA/694), CF405S conjugate

• Description: Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Cyclin; DNA polymerase delta auxiliary protein; Mutagen-sensitive 209 protein; PCNAR; Polymerase delta accessory protein
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: PCNA
• Gene ID: 5111
• NCBI Gene ID: 147433 & 728886
• UniProt: P12004
• Cellular Locus: Nucleus & cytoplasm
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant full length human PCNA protein
• Target Antigen: PCNA
• Clonality: Monoclonal
• Isotype: IgG2a κ
• Clone: PCNA/694
• Conjugation: CF405S
• Source: Animal
• Applications: Flow, intracellular (verified) | IHC, FFPE (verified) | WB (verified)
• Validated Applications: FC, IHC, FFPE, WB
• Field of Research: Cell cycle
• Positive Control: Tonsil or reactive lymph node
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 36 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.25-0.5 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Predicted to show broad species reactivity|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years