Anti-PCNA(PCNA/694), CF405S conjugate

• Description:

  Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Cyclin; DNA polymerase delta auxiliary protein; Mutagen-sensitive 209 protein; PCNAR; Polymerase delta accessory protein

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  PCNA

• Gene ID:

  5111

• NCBI Gene ID:

  147433 & 728886

• UniProt:

  P12004

• Cellular Locus:

  Nucleus & cytoplasm

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant full length human PCNA protein

• Target Antigen:

  PCNA

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  PCNA/694

• Conjugation:

  CF405S

• Source:

  Animal

• Applications:

  Flow, intracellular (verified) | IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  FC, IHC, FFPE, WB

• Field of Research:

  Cell cycle

• Positive Control:

  Tonsil or reactive lymph node

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  36 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.25-0.5 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Predicted to show broad species reactivity|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4