P53 (DO-7), 0.2mg/mL

• Description :

  Recognizes a 53 kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. Its epitope maps within the N-terminus (aa 37-45) of p53. Monoclonal antibody PAb1801 does not block the binding of DO-7 MAb to p53 in an ELISA test. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms :

  Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53

• UNSPSC :

  41116161

• UNSPSC Description :

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name :

  TP53

• Gene ID :

  7157

• NCBI Gene ID :

  654481

• UniProt :

  P04637

• Swiss Prot :

  P04637

• Host :

  Mouse

• Species Reactivity :

  Cow, Human, Monkey

• Reactivity :

  P53 Tumor Suppressor Protein

• Immunogen :

  Recombinant human wild type p53 protein expressed in E. coli.

• Target Antigen :

  p53 Tumor Suppressor Protein

• Clonality :

  Monoclonal

• Clone :

  DO-7

• Type :

  Primary

• Source :

  Animal

• Origin :

  Animal

• Field of Research :

  Apoptosis, Tumor suppressors

• Positive Control :

  MDA-MB-231 Cells. Breast or Colon carcinoma

• Stability :

  2 years

• Concentration :

  0.2 mg/mL

• Buffer :

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight :

  53 kDa

• References & Citations :

  Vojtesek B et al. 1992. J. Immunol. Methods. 151(1-2): 237-44. | Stephen CW et al. 1995. J Mol. Biol. 248(1): 58-78. |

• Shipping Conditions :

  RT (ND)

• Storage Conditions :

  Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life :

  Guaranteed for at least 24 months from date of receipt when stored as recommended

• Fragment :

  IgG2b, kappa

• Species :

  Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

• Classification :

  PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

• CAS Number :

  9007-83-4

• Conjugate :

  Purified, with BSA