Anti-p21 / WAF1(WA-1)

Anti-p21 / WAF1(WA-1)

• Description: This MAb recognizes a 21 kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). In ELISA, MAb WA-1 is useful either as a solid phase or for detection of p21 protein.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Activating Fragment 1; CAP20; CDK-interacting protein 1; CDKI; CDKN1; CDKN1A; CIP1; Cyclin-dependent kinase inhibitor 1A (p21; Cip1); DNA Synthesis Inhibitor; MDA6; Melanoma Differentiation Associated Protein 6; p21Cip1/Waf1; PIC1; SDI1; SLC12A9; Wild type p53 activated fragment 1 (WAF1)
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CDKN1A
• Gene ID: 1026
• NCBI Gene ID: 370771
• UniProt: P38936
• Cellular Locus: Nucleus
• Host: Mouse
• Species Reactivity: Chimpanzee, Human, Monkey, Mouse, Rat
• Immunogen: Human recombinant p21 protein
• Target Antigen: p21 | WAF1
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: WA-1
• Conjugation: Purified, with BSA
• Source: Animal
• Applications: Flow, intracellular (published) | IF (published) | IHC, FFPE (verified) | WB (published)
• Validated Applications: FC, IF, IHC, FFPE, WB
• Field of Research: Cell cycle, Tumor suppressors
• Positive Control: HeLa Cells. Skin, colon, or breast carcinoma.
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 21 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. J Biol Chem (2001) 276(14): 11143-11150. (Flow, intracellular; IF) EMBO J (2002) 21:6771-6780. (WB)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years