Anti-Neurofilament (NF-L)(NR-4)

Anti-Neurofilament (NF-L)(NR-4)

• Description: This MAb reacts with a 68 kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: NEFH; Neurofilament H; Neurofilament Heavy Polypeptide 200kDa; Neurofilament Triplet H Protein; NF-H; NF200
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: NEFH
• Gene ID: 4747
• NCBI Gene ID: 521461
• UniProt: P07196
• Cellular Locus: Cytoskeleton
• Host: Mouse
• Species Reactivity: Chicken, Cow, Human, Pig, Rat
• Immunogen: Crude neurofilament preparation from porcine spinal cord
• Target Antigen: Neurofilament
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: NR-4
• Conjugation: Purified, with BSA
• Disease: Tumor
• Source: Animal
• Applications: Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified)
• Validated Applications: FC, IF, IHC, FFPE
• Field of Research: Cancer, Cytoskeleton, Neuroscience
• Positive Control: Brain, Neuroblastoma
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 68 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.25-0.5 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years