Anti-Muscle Specific Actin(HHF35)

Anti-Muscle Specific Actin(HHF35)

• Description: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: CAGH3; ERDA3; GDN; MAML3; Mastermind-like protein 3; TNRC3
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene ID: 58, 59 & 70
• NCBI Gene ID: 118127, 1288 & 500483
• UniProt: P62736, P68032; P68133
• Cellular Locus: Cytoskeleton
• Host: Mouse
• Species Reactivity: Cat, Chicken, Dog, Human, Mouse, Rabbit, Rat
• Immunogen: SDS extract of human myocardium.
• Target Antigen: Muscle Specific Actin
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: HHF35
• Conjugation: Purified, BSA-free
• Disease: Tumor
• Source: Animal
• Applications: IHC, FFPE (verified) | WB (verified)
• Validated Applications: IHC, FFPE, WB
• Field of Research: Cytoskeleton
• Positive Control: Muscle or sarcoma
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: Multiple
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.25-0.5 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years