Anti-Mucin 1 / EMA / Episialin / CD227(VU-2G7), CF594 conjugate

Anti-Mucin 1 / EMA / Episialin / CD227(VU-2G7), CF594 conjugate

• Description: MAb VU-2G7 reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of VU-2G7 has not been established with epitope fingerprinting. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Breast carcinoma-associated antigen DF3; CA15-3; Carcinoma-associated mucin Episialin; Epithelial Membrane Antigen; H23AG; KL-6; MAM6; MUC-1; MUC-1/SEC; MUC-1/X; MUC1-alpha; MUC1-beta; MUC1-CT; MUC1-NT; MUC1/ZD; Mucin 1 cell surface associated; Mucin-1 subunit beta; Peanut-reactive urinary mucin; PEM; PEMT; Polymorphic epithelial mucin; PUM; Tumor-associated epithelial membrane antigen
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: MUC1
• Gene ID: 4582
• NCBI Gene ID: 89603
• UniProt: P15941
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Synthetic glycosylated MUC1 60mer tandem repeat NH2-(HGVTSAPDT(GalNAc)RPAPGSTAPPAHG)3- COOH, conjugated to bovine serum albumin
• Target Antigen: CD227 | EMA | Episialin | Mucin 1
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: VU-2G7
• Conjugation: CF594
• Disease: Tumor
• Source: Animal
• Applications: Flow, surface (published) | IHC, FFPE (published) | ELISA (published)
• Validated Applications: FC, IHC, FFPE, ELISA
• Field of Research: Cancer, Mucins
• Positive Control: MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 265-400 kDa
• Additionnal Information: Immunohistology formalin-paraffin 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 1-2 ug/mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. ACS Omega (2017) 2 (11), 7493-7505. (ELISA, Flow) Arch Dermatol Res (2003) 295:146-154. (immunohistochemistry)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years