Anti-MUC18 / Mucin 18 / CD146(OJ79c), CF640R conjugate

Anti-MUC18 / Mucin 18 / CD146(OJ79c), CF640R conjugate

• Description: The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2 -independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease._x000D__x000D_Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_
• Synonyms: Cell Surface Glycoprotein MUC18; Cell Surface Glycoprotein P1H12; Gicerin; Melanoma Adhesion Molecule (MCAM); Melanoma Associated Glycoprotein MUC18; Melanoma Cell Adhesion Molecule; Melanoma-associated Antigen A32; Mel-CAM; S-endo 1 Endothelial-associated Antigen; Sendo1
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: MCAM
• Gene ID: 4162
• NCBI Gene ID: 599039
• UniProt: P43121
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant human MUC18 protein
• Target Antigen: CD146 | MCAM | MUC18 | Mucin 18
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: OJ79c
• Conjugation: CF640R
• Disease: Tumor
• Source: Animal
• Applications: Flow, surface (published) | IF (published) | ELISA (published)
• Validated Applications: FC, IF, ELISA
• Field of Research: Cancer, Mucins
• Positive Control: A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 130 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 0.5-1.0 ug/mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Microvascular Res (2009)78(3): 325-331. (ELISA) Stem Cells (2008) 26: 2408-2418. (Flow) Stem Cells and Development (2017) 26(13): 964. (IF)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years