Anti-MUC18 / Mucin 18 / CD146(OJ79c), CF488A conjugate

• Description:

  The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2 -independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease._x000D__x000D_Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_

• Synonyms:

  Cell Surface Glycoprotein MUC18; Cell Surface Glycoprotein P1H12; Gicerin; Melanoma Adhesion Molecule (MCAM); Melanoma Associated Glycoprotein MUC18; Melanoma Cell Adhesion Molecule; Melanoma-associated Antigen A32; Mel-CAM; S-endo 1 Endothelial-associated Antigen; Sendo1

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  MCAM

• Gene ID:

  4162

• NCBI Gene ID:

  599039

• UniProt:

  P43121

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human MUC18 protein

• Target Antigen:

  CD146 | MCAM | MUC18 | Mucin 18

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  OJ79c

• Conjugation:

  CF488A

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  Flow, surface (published) | IF (published) | ELISA (published)

• Validated Applications:

  FC, IF, ELISA

• Field of Research:

  Cancer, Mucins

• Positive Control:

  A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  130 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 0.5-1.0 ug/mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Microvascular Res (2009)78(3): 325-331. (ELISA) Stem Cells (2008) 26: 2408-2418. (Flow) Stem Cells and Development (2017) 26(13): 964. (IF)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years