Anti-MHC II (HLA-DRA)(IPO-10), CF640R conjugate

• Description: MAb IPO-10 defines the antigen that appears on B cell progenitors following HLA-DR and preceding CD10, CD19, CD22, CD37 and cym. It is expressed on resting B cells and than reappears and persists in cytoplasm and on cell surface until cytoplasmic Ig appears. It is a useful antibody for diagnostics of neoplasms of B cell origins. It reacts with human B cell lines Daudi, Raji, Namalva, EB-3, RPMI-8226 (50% of cells). The MAb does not label T cell lines, blood granulocytes, thymocytes or bone marrow stromal fibroblasts. No significant changes are detected after PHA or ConA stimulation while LPS and PWM stimulated cultures after 18-48h show decreased number of antigen-positive cells but in final terms of cultivation antigen is expressed again. This MAb labels B cell leukemias and some lymphomas. Hairy cell leukemia strongly reacts and 70% of B cell CLL and some B-NHL were also positive. IPO-10 reacts with AMML cells and in a majority of Hodgkin's disease cases a significant percentage of affected lymph node cells were detected. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Ia3; Ia-3; H-2Ea; H2-Ea; AI323765; I-Ealpha; E-alpha-f; MHC-H2-Ea
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: HLA-DRA
• Gene ID: 3122
• NCBI Gene ID: 520048
• UniProt: P01903
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human, Monkey
• Immunogen: Spleen cells of a patient with hairy cell leukemia (Daudi cells)
• Target Antigen: MHC-II
• Clonality: Monoclonal
• Isotype: IgG3 κ
• Clone: IPO-10
• Conjugation: CF640R
• Source: Animal
• Field of Research: Immunology, MHC antigens
• Positive Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 36 kDa
• Additionnal Information: For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Anticancer Res (1988) 8(6):1377-1380. Tissue Antigens (1989) 33: 151. Neoplasma (1991) 39(1):3-9.
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years