Anti-MHC II (HLA-DQ)(SPV-L3), CF640R conjugate

Anti-MHC II (HLA-DQ)(SPV-L3), CF640R conjugate

• Description: Recognizes a DQ antigen, which is a dimer of 60 kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: Ia3; Ia-3; H-2Ea; H2-Ea; AI323765; I-Ealpha; E-alpha-f; MHC-H2-Ea
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene ID: 3117; 3118; 3119
• NCBI Gene ID: 550475
• UniProt: P01908; P01909; P01920
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human, Pig
• Immunogen: T4-positive CTL clone HG-38
• Target Antigen: MHC-II
• Clonality: Monoclonal
• Isotype: IgG2a κ
• Clone: SPV-L3
• Conjugation: CF640R
• Source: Animal
• Applications: Functional studies (published) | Flow, surface (verified) | IF (verified) | IP (published) | WB (published)
• Validated Applications: FC, IF, IP, WB
• Field of Research: Immunology, MHC antigens
• Positive Control: Raji cells. Tonsil or lymph node
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 60 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Hybridoma (1983) 2(4):423-37. (functional studies) Eur J Immunol (1984) 14: 299-304. (immunoprecipitation) J Biol Chem (2010) 285(52): 40800-40808. (western)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years