Anti-HLA-A (MHC I) (108-2C5)

Anti-HLA-A (MHC I) (108-2C5)

• Description: HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation. This MAb is useful for HLA molecular typing of peripheral blood leukocytes as well as a large number of leukemic cell lines. It reacts with an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, -A28, -A29, -A30, -A31 and -Aw33). Its epitope maps between aa65-to-aa80 of thealpha1 domain of the HLA-A. This MAb recognizes an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, A28, -A29, -A30, -A31 and -Aw33). Furthermore, by testing its reactivity with HLA-A2 natural variants and mutants, the importance of amino acid residues 79 and/or 80 of thealpha1 domain was demonstrated in the formation of an intralocus HLA-A determinant.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: HLA-A; HLA class 1A; MHC class I HLA-A
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: HLA-A
• Gene ID: 3105
• NCBI Gene ID: 181244; 654404; 77961
• UniProt: P01889; P30443; P30499
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Normal human peripheral blood lymphocytes
• Target Antigen: HLA-A
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: 108-2C5
• Conjugation: Purified, with BSA
• Source: Animal
• Applications: Flow (verified) | Flow, surface (published) | IF (published) | IHC, frozen (published) | IP (published)
• Validated Applications: FC, FC, IF, IHC, IP
• Field of Research: Cancer, Immunology, MHC antigens
• Positive Control: THP-1 and Jurkat cells. Tonsil or lymph node.
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: ~41 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Tissue Antigens (2004) 64: 696-702. (IHC, frozen) Clin Cancer Res (2005) 11: 498-504. (IHC, frozen) Clin Exp Immunol (2006) 145: 365-371. (IF) Mol Cell Proteom (2015) 14(7): 1770-1780. (Flow, surface; IP)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years