Anti-CD3e(B-B12), CF740 conjugate

Anti-CD3e(B-B12), CF740 conjugate

• Description: Reacts with five invariable CD3 chains (designated as and ) with molecular weight ranging from 16-28 kDa. CD3 is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. The CD3 complex is closely associated at the lymphocyte cell surface with T cell antigen receptor (TCR) and is involved in transducing antigen-recognition signals into cytoplasm of T cells and in regulating the cell surface expression of the TCR complex._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: CD 3E|CD3 epsilon|CD3 TCR complex|CD3E|CD3e antigen epsilon polypeptide (TiT3 complex)|T cell antigen receptor complex epsilon subunit of T3|T-cell surface antigen T3/Leu-4 epsilon chain|T-cell surface glycoprotein CD3 epsilon chain|T3E|TCRE|TiT3 complex
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CD3E
• Gene ID: 916
• NCBI Gene ID: 3003
• UniProt: P07766
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Human T cell leukemia cells
• Target Antigen: CD3e
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: B-B12
• Conjugation: CF740
• Disease: Tumor
• Source: Animal
• Applications: Flow, surface (verified) | IF (verified)
• Validated Applications: FC, IF
• Field of Research: Immunology
• Positive Control: Jurkat cells. Tonsil and lymph node
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% rBSA, 0.05% azide
• Molecular Weight: 20 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Kishimoto T. et al. eds. Leukocyte Typing VI, p44-48 and p1111, Garland Publishing, Inc, New York and London, 1997.
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years