Anti-Human Kappa Light Chain(L1C1), CF640R conjugate

Anti-Human Kappa Light Chain(L1C1), CF640R conjugate

• Description: This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of kappa to lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: HCAK1; Ig Kappa Chain C Region; IGKC; Immunoglobulin KM
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: IGKC
• Gene ID: 3514
• NCBI Gene ID: 449609
• UniProt: P01601; P01834
• Cellular Locus: Plasma membrane|Secreted (extracellular)
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Human B-Lymphoma Cells
• Target Antigen: Human Kappa Light Chain
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: L1C1
• Conjugation: CF640R
• Disease: Tumor
• Source: Animal
• Applications: IHC, FFPE (verified) | WB (verified)
• Validated Applications: IHC, FFPE, WB
• Field of Research: Cancer, Immunology, Inflammation
• Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: ~22.5 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Does not react with rat; others not known|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Western blotting 0.5-1 ug/mL|Flow Cytometry 0.5-2.0 ug/million cells in 0.1 mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years