Anti-Insulin(2D11-H5)

Anti-Insulin(2D11-H5)

• Description: Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: IDDM2, ILPR, IRDN, MODY10, Proinsulin
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: INS
• Gene ID: 3630
• NCBI Gene ID: 272259
• UniProt: P01308
• Cellular Locus: Secreted (extracellular)
• Host: Mouse
• Species Reactivity: Cow, Human, Mouse, Pig
• Immunogen: Purified pig insulin, conjugated to KLH
• Target Antigen: Insulin
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: 2D11-H5
• Conjugation: Purified, with BSA
• Source: Animal
• Applications: IF (published) | IHC, FFPE (verified)
• Validated Applications: IF, IHC, FFPE
• Field of Research: Endocrinology
• Positive Control: MIA PaCa-2 cells or Pancreas
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 6 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.1-0.2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. J Biol Chem (2007) 282(46): 33583-33592. (IHC, FFPE) Histochem Cell Biol (2020) 153: 279-285. (IF)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years