Biotinylated Human Frizzled-8 (C-6His-Avi)
CAT:
855-EPT227
Size:
100 μg
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No

Biotinylated Human Frizzled-8 (C-6His-Avi)
- Background: Frizzled-8 is one of at least ten seven-transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co-receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. These ligands bind the extracellular CRD of Frizzled-8, blocking Wnt binding. The recombinant Frizzled-8 CRD has also been used to block Wnt signaling and inhibit growth of teratocarcinomas.
- Description: Biotinylated Recombinant Human Frizzled-8 is produced by our Mammalian expression system and the target gene encoding Ala28-Pro172 is expressed with a 6His, Avi tag at the C-terminus.
- Synonyms: frizzled 8; frizzled family receptor 8; Frizzled8; Frizzled-8; FZ-8; FZD8; hFZ8
- UniProt: Q9H461
- Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
- Purity: Greater than 95% as determined by reducing SDS-PAGE.
- Molecular Weight: 19.1 KDa
- Shipping Conditions: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
- Storage Conditions: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.