Recombinant Mouse B7-H4 (C-Fc)

Recombinant Mouse B7-H4 (C-Fc)

• Background: Mouse V-set domain-containing T-cell activation inhibitor 1/VTCN1/B7-H4 is glycosylated member of the B7 family of immune co‑stimulatory proteins. B7-H4 consists of extracellular domain (ECD) with one Ig-like V-set domain and one Ig-like C2-set domain. It is widely expressed, including in kidney, liver, lung, pancreas, placenta, prostate, spleen, testis and thymus. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. It also involved in promoting epithelial cell transformation.
• Description: Recombinant Mouse B7 Homolog 4 is produced by our Mammalian expression system and the target gene encoding Phe29-Pro258 is expressed with a Fc tag at the C-terminus.
• Synonyms: V-set domain containing T-cell activation inhibitor 1; B7 homolog 4; Protein B7S1; B7-H4; VTCN1
• CAS Number: 9000-83-3
• UniProt: AAH32925.1
• Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Bioactivity: Immobilized Anti-Human B7-H4 mAb(Cat#NC054) at 2μg/ml (100 μl/well) can bind Mouse B7-H4-Fc*(Cat#CS04).*: Biotinylated by NHS-biotin prior to testing The ED50 of Mouse B7-H4-Fc*(Cat#CS04) is 4.11 ng/ml.
• Molecular Weight: 52.2 KDa
• Shipping Conditions: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
• Storage Conditions: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.