Recombinant Mouse IL-10

Recombinant Mouse IL-10

• Background: Mouse Il10 is the prototypic member of the IL-10 cytokine family, including IL-10, IL-19, IL-20, IL-22 (IL-TIF), IL-24 and IL-26. Many viruses encode viral members of the IL-10 family, such as Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV).Its main function is inhibiting the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Although human and mouse IL-10 are 81% identical at the nucleotide and amino acid level, mouse IL-10 is species-specific and does not act on human cells. Interestingly, Human IL-10 is active on mouse cells.
• Description: Recombinant Mouse Interleukin-10 is produced by our E.coli expression system and the target gene encoding Ser19-Ser178 is expressed.
• Synonyms: Interleukin-10; Il10; IL-10; Cytokine synthesis inhibitory factor; CSIF
• CAS Number: 9000-83-3
• UniProt: P18893
• Endotoxin: Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Bioactivity: Measured in a cell proliferation assay using FDC-P1 Mouse bone marrow cells The ED50 for this effect is 6 ng/ml.
• Molecular Weight: 18.9 KDa
• Shipping Conditions: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
• Storage Conditions: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.