Anti-IgA Secretory Component(SC05), CF405S conjugate

Anti-IgA Secretory Component(SC05), CF405S conjugate

• Description: This MAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: ECM1, Extracellular Matrix Protein 1, Secretory Component p85, URBWD
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: ECM1
• Gene ID: 1893
• NCBI Gene ID: 81071
• UniProt: Q16610
• Cellular Locus: Secreted (extracellular)
• Host: Mouse
• Species Reactivity: Human, Rat
• Immunogen: Secretory Component protein isolated from human colostrum
• Target Antigen: IgA Secretory Component
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: SC05
• Conjugation: CF405S
• Source: Animal
• Applications: Functional studies (published) | IHC, FFPE (verified) | WB (published)
• Validated Applications: IHC, FFPE, WB, Functional assays
• Field of Research: Cancer, Immunology, Inflammation
• Positive Control: Stomach, Lung, or breast tumor
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: ~80 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Infection Immunity (2010)78(11): 4589-4592. (WB) Clin Exp Immunol (2017) 195: 287-301. (functional studies)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years