Anti-HSP27(G3.1)

Anti-HSP27(G3.1)

• Description: This MAb reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian α-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore HSP27 may be useful in classifying various hormone sensitive tumors._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: Heat shock 27kDa protein; Estrogen-regulated 24kDa protein; Heat shock 25kDa protein 1; Heat shock 28kDa protein 1; Heat shock protein beta-1; HMN2B; Hsp25; Hsp28; HspB1; Stress-responsive protein 27 (SRP27)
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: HSPB1
• Gene ID: 3315
• NCBI Gene ID: 520973
• UniProt: P04792
• Cellular Locus: Cytoplasmic|Plasma membrane
• Host: Mouse
• Species Reactivity: Chicken, Chimpanzee, Human, Monkey, Mouse, Rat, Sheep
• Immunogen: Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
• Target Antigen: HSP27
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: G3.1
• Conjugation: Purified, BSA-free
• Source: Animal
• Applications: Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified) | IP (published) | WB (verified)
• Validated Applications: FC, IF, IHC, FFPE, IP, WB
• Field of Research: Endocrinology, Heat shock proteins
• Positive Control: ~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR.
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: 27 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.25-0.5 ug/mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Nat Comm (2018) 9: 1431. (immunoprecipitation; IHC, FFPE; flow, intracellular, proximity ligation assay)
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years