Anti-HLA-DRB(HLA-DRB/1067), Biotin conjugate

• Description:

  This MAb reacts with the beta-chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Its epitope is different from that of MAb L243. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4 T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  DRB1; HLA class II histocompatibility antigen; DR-1 beta chain; HLA-DR-beta 1; HLA-DRB1; human leucocyte antigen DRB1; Leucocyte antigen DR beta 1 chain; lymphocyte antigen DRB1; major histocompatibility complex; class II; DR beta 1; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR-beta cell surface glycoprotein

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  HLA-DRB1

• Gene ID:

  3123

• NCBI Gene ID:

  534322

• UniProt:

  P01911

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human, Monkey

• Immunogen:

  Activated human peripheral blood mononuclear cells

• Target Antigen:

  HLA-DRB

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  HLA-DRB/1067

• Conjugation:

  Biotin

• Source:

  Animal

• Applications:

  Flow (verified) | IF (verified) | IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  FC, IF, IHC, FFPE, WB

• Field of Research:

  Cancer, Immunology, MHC antigens

• Positive Control:

  Ramos, Daudi or HuT78 cells. Tonsil or lymph node

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  28 kDa (beta chain)

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.25-0.5 ug/mL|Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4