Anti-HCG-intact(HCGab/52), CF640R conjugate

Anti-HCG-intact(HCGab/52), CF640R conjugate

• Description: This MAb is a very special because it reacts ONLY with the intact-HCG and not with either free alpha- or free beta-chain of HCG. HCG is a glycoprotein and is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: CG-alpha; CGA; Chorionic Gonadotrophin Alpha; Follicle Stimulating Hormone Alpha; Follitropin Alpha; FSH-alpha; FSHA; GPH Alpha; GPHA1; LHA; LH-alpha; Luteinizing Hormone Alpha; Lutropin Alpha; Thyroid Stimulating Hormone Alpha; Thyrotropin Alpha; TSHA
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CGA, CGB3
• Gene ID: 1081 & 1082
• NCBI Gene ID: 172944 & 119689
• UniProt: P01215; P01233
• Cellular Locus: Secreted (extracellular)|Vesicular
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Purified hCG protein
• Target Antigen: HCG intact
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: HCGab/52
• Conjugation: CF640R
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Endocrinology
• Positive Control: JAR or TT Cells. Placenta
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: ~35 kDa (intact hCG)
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years