Anti-Golgi Complex(371-4), CF405S conjugate

Anti-Golgi Complex(371-4), CF405S conjugate

• Description: This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Golgi Complex; Golgi apparatus; Golgi zone
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene ID: Not Known
• NCBI Gene ID: Not Known
• UniProt: Not Known
• Cellular Locus: Golgi apparatus
• Host: Mouse
• Species Reactivity: Human
• Immunogen: SU-DHL-1 large cell lymphoma cells.
• Target Antigen: Golgi Complex
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: 371-4
• Conjugation: CF405S
• Source: Animal
• Applications: IF (published) | IHC, FFPE (verified)
• Validated Applications: IF, IHC, FFPE
• Field of Research: Organelle markers
• Positive Control: HepG2, A431 or HeLa cells. Placenta, Tonsil, Testis and Ovary.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: Not Known
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Does not react with mouse or rat, others not known|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Immunocytochemistry Acetone or paraformaldehyde fixed 0.5-1 ug/mL|Western blotting 0.5-1.0 ug/mL|Flow Cytometry 0.5-1.0 ug/million cells in 0.1 mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Cancer Res (2001) 61: 3339-3347. (IF) J Virol (2005) 79(13): 8506. (IF)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years