Flag-Tag Mouse mAb
CAT:
855-EM1000-01
Size:
50 μL
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Flag-Tag Mouse mAb
- Description: The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
- Host: Mouse
- Immunogen: Synthetic Peptide
- Clonality: Monoclonal
- Validated Applications: WB, IP, IF
- Stability: 1 year
- Concentration: 1 mg/mL
- Dilution: WB 1:5,000 IP 1:200 IF 1:2,000
- Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.