Anti-Glycophorin A / CD235a(GPHN02)

• Description:

  Recognizes a sialoglycoprotein of 39 kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

• Synonyms:

  Blood group--MN locus; GPA; GPErik; GpMiIII; GPSAT; GYPA; MN sialoglycoprotein; MNS; PAS2; Sialoglycoprotein alpha

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  GYPA

• Gene ID:

  2993 & 2994

• NCBI Gene ID:

  434973; 654368

• UniProt:

  P02724

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Cow, Human

• Immunogen:

  Human erythrocytes treated with neuraminidase

• Target Antigen:

  CD235a | Glycophorin-A

• Clonality:

  Monoclonal

• Isotype:

  IgM κ

• Clone:

  GPHN02

• Conjugation:

  Purified, BSA-free

• Source:

  Animal

• Field of Research:

  Hematology

• Positive Control:

  Erythrocytes

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  39 kDa

• Additionnal Information:

  For coating for ELISA, order Ab without BSA|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry

• References & Citations:

  Cartron JP and Rahuel C. Human erythrocyte glycophorins: protein and gene structure analyses. Transfus Med Rev 1992,6(2):63-92 | Gahmberg CG et al. Biosynthesis of the major human red cell sialoglycoprotein, glycophorin A. A review. Rev Fr Transfus Immunohematol 1981,24(1):53-73 | Wybenga LE et al. Glycophorin as a receptor for Sendai virus. Biochemistry 1996, 35(29):9513-8 | Rahuel C et al. Post-transcriptional regulation of the cell surface expression of glycophorins A, B, and E. J Biol Chem 1994, 269(52):32752-8 | Thacker TC and Johnson FB. Binding of bovine parvovirus to erythrocyte membrane sialylglycoproteins. J Gen Virol 1998, 79:2163-

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4