Anti-Fibronectin(HFN7.1), CF640R conjugate

• Description: Fibronectin is a dimeric glycoprotein of 440 kDa, which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. This MAb is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Cold insoluble globulin (CIG); FINC; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor (MSF); Ugl-Y3
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: FN1
• Gene ID: 2335
• NCBI Gene ID: 203717
• UniProt: P02751
• Cellular Locus: Extracellular matrix
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Human fibronectin purified from serum by affinity chromatography on gelatin-sepharose
• Target Antigen: Fibronectin
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: HFN7.1
• Conjugation: CF640R
• Source: Animal
• Applications: Functional studies (published) | IF (published) | ELISA (published) | IHC, FFPE (verified) | WB (published)
• Validated Applications: IF, ELISA, IHC, FFPE, WB
• Field of Research: Cell adhesion, Extracellular matrix
• Positive Control: SW156 cells or Kidney
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 220 kDa (monomer); 440 kDa (dimer)
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. J Biol Chem (1991) 266(14): 23323-23328. (inhibition of integrin binding; epitope mapping) Anat Rec (1996) 245:068-676. (IF, frozen tissue) PNAS USA (2005) 102(17): 5953-5957. (functional studies) Biomaterials (2005) 26: 4523-4531. (IF; functional studies) Hybridoma (2006) 25(4): 202-208. (ELISA; WB)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years