Recombinant Mycobacterium smegmatis 3-oxosteroid 1-dehydrogenase (ksdD)

• CAS Number:

  9000-83-3

• Gene Name:

  ksdD

• UniProt:

  A0R4S9

• Expression Region:

  1-569aa

• Organism:

  Mycolicibacterium smegmatis (strain ATCC 700084 / mc (2)155) (Mycobacterium smegmatis)

• Target Sequence:

  MFYMTGQEYDVVVVGSGAAGMVAALTAAHQGLSTVVVEKAPHYGGSTARSGGGVWIPNNEILKRDGVKDTPDEARKYLHAIIGDVVPAEKIDTYLDRGPEMLSFVLKHSPLKLCWVPGYSDYYPETPGGKPTGRSVEPKPFDANKLGPDLKGLEPPYGKVPMNMVVMQQDYVRLNQLKRHPRGVLRSLKVGIRATWGKVSGKNLVGMGRALIAPLRIGLREAGVPVLLNTALTDLYVEDGRVLGIYVRDTTAGDDAEPRLIRARHGVILGSGGFEHNEQMRVKYQRAPITTEWTVGAVANTGDGIVAAEKLGAALELMEDAWWGPTVPLVGAPWFALSERNSPGSIIVNMSGKRFMNESMPYVEACHHMYGGQYGQGPGPGENIPAWLIFDQQYRDRYIFAGLQPGQRIPSKWLESGVIVKADTLVELAEKTGLPADQFTSTIERFNGFARSGVDEDFHRGESAYDRYYGDPTNKPNPNLGEIKHGPFYAAKMVPGDLGTKGGIRTDVRGRALRDDDSVIEGLYAAGNVSSPVMGHTYPGPGGTIGPAMTFGYLAALDIAATAAASRKG

• Tag:

  N-terminal 10xHis-tagged and C-terminal Myc-tagged

• Source:

  E.coli

• Field of Research:

  Others

• Assay Type:

  In Stock Protein

• Relevance:

  Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3-ketosteroids. Is also involved in the formation of 1, 4-androstadiene-3, 17-dione (ADD) from 4-androstene-3, 17-dione (AD) to.

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Length:

  Full Length

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  68.9 kDa

• References & Citations:

  "Identification and targeted disruption of the gene encoding the main 3-ketosteroid dehydrogenase in Mycobacterium smegmatis." Brzostek A., Sliwinski T., Rumijowska-Galewicz A., Korycka-Machala M., Dziadek J. Microbiology 151:2393-2402 (2005)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.