Recombinant Canine distemper virus Nucleoprotein (N) , partial

Recombinant Canine distemper virus Nucleoprotein (N) , partial

• CAS Number: 9000-83-3
• Gene Name: N
• UniProt: P04865
• Expression Region: 399-523aa
• Organism: Canine distemper virus (strain Onderstepoort) (CDV)
• Target Sequence: SKTTEDRTIRATGPKQSQITFLHSERSEVANQQPPTINKRSENQGGDKYPIHFSDERLPGYTPDVNSSEWSESRYDTQIIQDDGNDDDRKSMEAIAKMRMLTKMLSQPGTSEDNSPVYSDKELLN
• Tag: C-terminal 6xHis-Myc-tagged
• Source: Yeast
• Field of Research: Microbiology
• Assay Type: Developed Protein
• Relevance: Encapsidates the genome in a ratio of 1 N per 6 ribonucleotides, protecting it from nucleases. The nucleocapsid (NC) has a helical structure. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity).
• Purity: Greater than 85% as determined by SDS-PAGE.
• Activity: Not Test
• Length: Partial
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 17.9 kDa
• References & Citations: "Canine distemper terminal and intergenic non-protein coding nucleotide sequences: completion of the entire CDV genome sequence." Sidhu M.S., Husar W., Cook S.D., Dowling P.C., Udem S.A. Virology 193:66-72 (1993)
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.