Anti-Double Stranded DNA (dsDNA)(121-3), CF647 conjugate

Anti-Double Stranded DNA (dsDNA)(121-3), CF647 conjugate

• Description: This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This MAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells.,Deoxyribonucleic acid (DNA) is a long polymer of nucleotides that is held together by a backbone made of sugars and phosphate groups. It holds the genetic instructions for the development and function of living things. DNA is crucial for living organisms, and all known cellular life and some viruses contain DNA. In eukaryotes, DNA exists in the cell nucleus, while in prokaryotes; DNA is located in the cytoplasm. In living organisms, DNA does not usually exist as a single molecule, but instead as a tightly associated pair of molecules in the shape of a right-handed double helix. Hydrogen bonds as well as forces generated by the hydrophobic effect and pi stacking hold the two DNA strands together. During replication and transcription, portions of the helix unwind and become single stranded. Protective proteins surround these single-stranded DNA. Double stranded (ds) DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Not Known
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene ID: Not Known
• NCBI Gene ID: Not Known
• UniProt: Not Known
• Cellular Locus: Nucleus
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Nuclei of Burkitt's cells
• Target Antigen: Double-Stranded DNA
• Clonality: Monoclonal
• Isotype: IgG3 κ
• Clone: 121-3
• Conjugation: CF647
• Source: Animal
• Applications: Flow, intracellular (verified) | IHC, FFPE (verified)
• Validated Applications: FC, IHC, FFPE
• Field of Research: Organelle markers
• Positive Control: Tonsil
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: Not Known
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years