Sheep T3 (Triiodothyronine) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sheep T3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep T3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep T3 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin

• Gene Aliases:

  3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin

• Reactivity:

  Sheep

• Field of Research:

  Endocrinology, Autoimmunity

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sheep T3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep T3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep T3 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2.5 H

• Standard:

  10 ng/mL

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  0.16 - 10 ng/mL

• Sensitivity:

  0.065 ng/mL

• Species:

  Sheep

• CAS Number:

  7732-18-5